Uncrewed aerial vehicle with onboard winch system for rapid, cost-effective, and safe oceanographic profiling in hazardous and inaccessible areas.

Interactions between coastal waters and marine-terminating glaciers in the Polar Regions play a significant role in global sea level rise fueled by a rapidly warming Arctic. The risk of glacier calving, and the abundance of ice, can make it impossible for surface vessels to access the waters near glacier termini. Alternative methods using manned aircraft are expensive. As a result, oceanographic measurements are limited near glacier termini. We present an uncrewed aerial vehicle (UAV) with an on-board winch system that allows oceanographic profiling in remote, hazardous areas using a commercial conductivity, temperature, and depth (CTD) sensor payload. The UAV is optimized for easy handling and deployment and is capable of high-speed and efficient cruise flight. An autopilot system provides pilot assistance and autonomous flight capabilities. The total weight of the UAV including payload is 6.5 kg with an endurance of 24 min. Testing of the system was conducted in South Greenland during winter conditions in March 2023 with successful profiles collected near a glacier terminus (<5 m) and in small openings in ice mélange (2.2 m). The system proved capable, reliable, and efficient. Further development of the system will allow other sensors for an even more flexible measurement suite.

Seafloor primary production in a changing Arctic Ocean.

Phytoplankton and sea ice algae are traditionally considered to be the main primary producers in the Arctic Ocean. In this Perspective, we explore the importance of benthic primary producers (BPPs) encompassing microalgae, macroalgae, and seagrasses, which represent a poorly quantified source of Arctic marine primary production. Despite scarce observations, models predict that BPPs are widespread, colonizing ~3 million km2 of the extensive Arctic coastal and shelf seas. Using a synthesis of published data and a novel model, we estimate that BPPs currently contribute ~77 Tg C y-1 of primary production to the Arctic, equivalent to ~20 to 35% of annual phytoplankton production. Macroalgae contribute ~43 Tg C y-1, seagrasses contribute ~23 Tg C y-1, and microalgae-dominated shelf habitats contribute ~11 to 16 Tg C y-1. Since 2003, the Arctic seafloor area exposed to sunlight has increased by ~47,000 km2 y-1, expanding the realm of BPPs in a warming Arctic. Increased macrophyte abundance and productivity is expected along Arctic coastlines with continued ocean warming and sea ice loss. However, microalgal benthic primary production has increased in only a few shelf regions despite substantial sea ice loss over the past 20 y, as higher solar irradiance in the ice-free ocean is counterbalanced by reduced water transparency. This suggests complex impacts of climate change on Arctic light availability and marine primary production. Despite significant knowledge gaps on Arctic BPPs, their widespread presence and obvious contribution to coastal and shelf ecosystem production call for further investigation and for their inclusion in Arctic ecosystem models and carbon budgets.

The effect of hydrostatic pressure on the activity and community composition of hydrocarbon-degrading bacteria in Arctic seawater.

IMPORTANCE: Increased ship traffic in the Arctic region raises the risk of oil spills. With an average sea depth of 1,000 m, there is a growing concern over the potential release of oil sinking in the form of marine oil snow into deep Arctic waters. At increasing depth, the oil-degrading community is exposed to increasing hydrostatic pressure, which can reduce microbial activity. However, microbes thriving in polar regions may adapt to low temperature by modulation of membrane fluidity, which is also a well-known adaptation to high hydrostatic pressure. At mild hydrostatic pressures up to 8-12 MPa, we did not observe an altered microbial activity or community composition, whereas comparable studies using deep-sea or sub-Arctic microbial communities with in situ temperatures of 4-5°C showed pressure-induced effects at 10-15 MPa. Our results suggest that the psychrophilic nature of the underwater microbial communities in the Arctic may be featured by specific traits that enhance their fitness at increasing hydrostatic pressure.

Glacier retreat alters downstream fjord ecosystem structure and function in Greenland.

The melting of the Greenland Ice Sheet is accelerating, with glaciers shifting from marine to land termination and potential consequences for fjord ecosystems downstream. Monthly samples in 2016 in two fjords in southwest Greenland show that subglacial discharge from marine-terminating glaciers sustains high phytoplankton productivity that is dominated by diatoms and grazed by larger mesozooplankton throughout summer. In contrast, melting of land-terminating glaciers results in a fjord ecosystem dominated by bacteria, picophytoplankton and smaller zooplankton, which has only one-third of the annual productivity and half the CO2 uptake compared to the fjord downstream from marine-terminating glaciers.

Long-term patterns of hydrocarbon biodegradation and bacterial community composition in epipelagic and mesopelagic zones of an Arctic fjord.

Oil spill attenuation in Arctic marine environments depends on oil-degrading bacteria. However, the seasonally harsh conditions in the Arctic such as nutrient limitations and sub-zero temperatures limit the activity even for bacteria capable of hydrocarbon metabolism at low temperatures. Here, we investigated whether the variance between epipelagic (seasonal temperature and inorganic nutrient variations) and mesopelagic zone (stable environmental conditions) could limit the growth of oil-degrading bacteria and lead to lower oil biodegradation rates in the epipelagic than in the mesopelagic zone. Therefore, we deployed absorbents coated with three oil types in a SW-Greenland fjord system at 10-20 m (epipelagic) and 615-650 m (mesopelagic) water depth for one year. During this period we monitored the development and succession of the bacterial biofilms colonizing the oil films by 16S rRNA gene amplicon quantification and sequencing, and the progression of oil biodegradation by gas chromatography - mass spectrometry oil fingerprinting analysis. The removal of hydrocarbons was significantly different, with several polycyclic aromatic hydrocarbons showing longer half-life times in the epipelagic than in the mesopelagic zone. Bacterial community composition and density (16S rRNA genes/ cm2) significantly differed between the two zones, with total bacteria reaching to log-fold higher densities (16S rRNA genes/cm2) in the mesopelagic than epipelagic oil-coated absorbents. Consequently, the environmental conditions in the epipelagic zone limited oil biodegradation performance by limiting bacterial growth.

Coastal freshening drives acidification state in Greenland fjords.

Greenland's fjords and coastal waters are highly productive and sustain important fisheries. However, retreating glaciers and increasing meltwater are changing fjord circulation and biogeochemistry, which may threaten future productivity. The freshening of Greenland fjords caused by unprecedented melting of the Greenland Ice Sheet may alter carbonate chemistry in coastal waters, influencing CO2 uptake and causing biological consequences from acidification. However, few studies to date explore the current acidification state in Greenland coastal waters. Here we present the first-ever large-scale measurements of carbonate system parameters in 16 Greenlandic fjords and seek to identify the drivers of acidification state in these freshening ecosystems. Aragonite saturation state (Ω), a proxy for ocean acidification, was calculated from dissolved inorganic carbon (DIC) and total alkalinity from fjords along the east and west coast of Greenland spanning 68-75°N. Aragonite saturation was primarily >1 in the surface mixed layer. However, undersaturated-or corrosive--conditions (Ω < 1) were observed on both coasts (west: Ω = 0.28-3.11, east: Ω = 0.70-3.07), albeit at different depths. West Greenland fjords were largely corrosive at depth while undersaturation in East Greenland fjords was only observed in surface waters. This reflects a difference in the coastal boundary conditions and mechanisms driving acidification state. We suggest that advection of Sub Polar Mode Water and accumulation of DIC from organic matter decomposition drive corrosive conditions in the West, while freshwater alkalinity dilution drives acidification in the East. The presence of marine terminating glaciers also impacted local acidification states by influencing fjord circulation: upwelling driven by subglacial discharge brought corrosive bottom waters to shallower depths. Meanwhile, discharge from land terminating glaciers strengthened stratification and diluted alkalinity. Regardless of the drivers in each system, increasing freshwater discharge will likely lower carbonate saturation states and impact biotic and abiotic carbon uptake in the future.

A mobile observatory powered by sun and wind for near real time measurements of atmospheric, glacial, terrestrial, limnic and coastal oceanic conditions in remote off-grid areas.

Climate change is rapidly altering the Arctic environment. Although long-term environmental observations have been made at a few locations in the Arctic, the incomplete coverage from ground stations is a main limitation to observations in these remote areas. Here we present a wind and sun powered multi-purpose mobile observatory (ARC-MO) that enables near real time measurements of air, ice, land, rivers, and marine parameters in remote off-grid areas. Two test units were constructed and placed in Northeast Greenland where they have collected data from cabled and wireless instruments deployed in the environment since late summer 2021. The two units can communicate locally via WiFi (units placed 25 km apart) and transmit near-real time data globally over satellite. Data are streamed live and accessible from (https://gios.org). The cost of one mobile observatory unit is c. 304.000€. These test units demonstrate the possibility for integrative and automated environmental data collection in remote coastal areas and could serve as models for a proposed global observatory system.

Lightweight drone-deployed autonomous ocean profiler for repeated measurements in hazardous areas - Example from glacier fronts in NE Greenland.

Accelerated melting of ice in Polar Regions due to global warming increases freshwater input to coastal waters from marine terminating glaciers. Lack of measurements near the glacier terminus limits our knowledge of the mixing processes between freshwater and the underlying ocean. We present a low-cost (< € 3200) and lightweight (2.6 kg) drone-deployed, retrievable conductivity, temperature and depth (CTD) instrument for remote controlled (1 km) autonomous profiling in highly hazardous and remote areas. The instrument was deployed with a drone taking off from land and marine vessels to perform measurements near tidewater glaciers termini of the Greenland ice sheet. The free-flowing profiler is reusable due to a compact ballast based single-shot buoyancy engine and post-profiling pickup by drone. It can reach a depth of up to 250 m, and is equipped with low-cost sensors for conductivity, temperature, and depth measurements. During decent the profiler reaches a velocity of about 0.48 m/s, resulting in about 3.5 data points pr. m depth, but is designed to easily vary the velocity by changing buoyancy setup before deployment. Successful tests were conducted at marine terminating glaciers in Northeast Greenland in August 2021.

Monitoring a changing Arctic: Recent advancements in the study of sea ice microbial communities.

Sea ice continues to decline across many regions of the Arctic, with remaining ice becoming increasingly younger and more dynamic. These changes alter the habitats of microbial life that live within the sea ice, which support healthy functioning of the marine ecosystem and provision of resources for human-consumption, in addition to influencing biogeochemical cycles (e.g. air-sea CO2 exchange). With the susceptibility of sea ice ecosystems to climate change, there is a pressing need to fill knowledge gaps surrounding sea ice habitats and their microbial communities. Of fundamental importance to this goal is the development of new methodologies that permit effective study of them. Based on outcomes from the DiatomARCTIC project, this paper integrates existing knowledge with case studies to provide insight on how to best document sea ice microbial communities, which contributes to the sustainable use and protection of Arctic marine and coastal ecosystems in a time of environmental change.

Biodegradation of water-accommodated aromatic oil compounds in Arctic seawater at 0 °C.

Oil spills in Arctic marine environments are expected to increase concurrently with the expansion of shipping routes and petroleum exploitation into previously inaccessible ice-dominated regions. Most research on oil biodegradation focusses on the bulk oil, but the fate of the water-accommodated fraction (WAF), mainly composed of toxic aromatic compounds, is largely underexplored. To evaluate the bacterial degradation capacity of such dissolved aromatics in Greenlandic seawater, microcosms consisting of 0 °C seawater polluted with WAF were investigated over a 3-month period. With a half-life (t1/2) of 26 days, m-xylene was the fastest degraded compound, as measured by gas chromatography - mass spectrometry. Substantial slower degradation was observed for ethylbenzene, naphthalenes, phenanthrene, acenaphthylene, acenaphthene and fluorenes with t1/2 of 40-105 days. Colwellia, identified by 16S rRNA gene sequencing, was the main potential degrader of m-xylene. This genus occupied up to 47 % of the bacterial community until day 10 in the microcosms. Cycloclasticus and Zhongshania aliphaticivorans, potentially utilizing one-to three-ringed aromatics, replaced Colwellia between day 10 and 96 and occupied up to 6 % and 23 % of the community, respectively. Although most of the WAF can ultimately be eliminated in microcosms, our results suggest that the restoration of an oil-impacted Arctic environment may be slow as most analysed compounds had t1/2 of over 2-3 months and the detrimental effects of a spill towards the marine ecosystem likely persist during this time.

Investigation into the geometry and distribution of oil inclusions in sea ice using non-destructive X-ray microtomography and its implications for remote sensing and mitigation potential.

As climate change brings reduced sea ice cover and longer ice-free summers to the Arctic, northern Canada is experiencing an increase in shipping and industrial activity in this sensitive region. Disappearing sea ice, therefore, makes the Arctic region susceptible to accidental releases of different types of oil and fuel pollution resulting in a pressing need for the development of appropriate scientific knowledge necessary to inform regulatory policy formulation. In this study, we examine the microstructure of the surficial layers of sea ice exposed to oil using X-ray microtomography. Through analysis, 3D imaging of the spatial distribution of the ice's components (brine, air, and oil) were made. Additional quantitative information regarding the size, proximity, orientation, and geometry of oil inclusions were computed to ascertain discernable relationships between oil and the other components of the ice. Our results indicate implications for airborne remote sensing and bioremediation of the upper sea ice layers.

An under-ice bloom of mixotrophic haptophytes in low nutrient and freshwater-influenced Arctic waters.

The pelagic spring bloom is essential for Arctic marine food webs, and a crucial driver of carbon transport to the ocean depths. A critical challenge is understanding its timing and magnitude, to predict its changes in coming decades. Spring bloom onset is typically light-limited, beginning when irradiance increases or during ice breakup. Here we report an acute 9-day under-ice algal bloom in nutrient-poor, freshwater-influenced water under 1-m thick sea ice. It was dominated by mixotrophic brackish water haptophytes (Chrysochromulina/ Prymnesium) that produced 5.7 g C m-2 new production. This estimate represents about half the annual pelagic production, occurring below sea ice with a large contribution from the mixotrophic algae bloom. The freshwater-influenced, nutrient-dilute and low light environment combined with mixotrophic community dominance implies that phagotrophy played a critical role in the under-ice bloom. We argue that such blooms dominated by potentially toxic mixotrophic algae might become more common and widespread in the future Arctic Ocean.

A cost-efficient low-weight autonomous profiler for measurements in polar coastal waters and other regions with strong density gradients.

The rapid warming of our planet has resulted in accelerated melting of ice in polar regions. Currently we have limited knowledge on how, where and when the surface meltwater layer is mixed with the underlying ocean due to lack of observations in these remote areas. We present a lightweight (17 kg) and low-cost (6000€) instrument for autonomous profiling across the strongly stratified upper layer in Arctic coastal waters, freshened by the riverine input and meltwater from glaciers, icebergs, and sea ice. The profiler uses a specially designed plunger buoyancy engine to displace up to 700 cm3 of water and allows for autonomous dives to 200 m depth. It can carry different sensor packages and convey its location by satellite communication. Two modes are available: (a) a free-floating mode and (b) a moored mode, where the instrument is anchored to the seafloor. In both modes, the profiler controls its velocity of 12 ± 0.3 cm/s resulting in 510 ± 22 data points per 100 m depth. Equipped with several sensors, e.g. conductivity, temperature, oxygen, and pressure, the autonomous profiler was successfully tested in a remote Northeast Greenlandic fjord. Data has been compared to traditional CTD instrument casts performed nearby.

Shells of the bivalve Astarte moerchi give new evidence of a strong pelagic-benthic coupling shift occurring since the late 1970s in the North Water polynya.

Climate changes in the Arctic may weaken the currently tight pelagic-benthic coupling. In response to decreasing sea ice cover, arctic marine systems are expected to shift from a 'sea-ice algae-benthos' to a 'phytoplankton-zooplankton' dominance. We used mollusc shells as bioarchives and fatty acid trophic markers to estimate the effects of the reduction of sea ice cover on the food exported to the seafloor. Bathyal bivalve Astarte moerchi living at 600 m depth in northern Baffin Bay reveals a clear shift in growth variations and Ba/Ca ratios since the late 1970s, which we relate to a change in food availability. Tissue fatty acid compositions show that this species feeds mainly on microalgae exported from the euphotic zone to the seabed. We, therefore, suggest that changes in pelagic-benthic coupling are likely due either to local changes in sea ice dynamics, mediated through bottom-up regulation exerted by sea ice on phytoplankton production, or to a mismatch between phytoplankton bloom and zooplankton grazing due to phenological change. Both possibilities allow a more regular and increased transfer of food to the seabed. This article is part of the theme issue 'The changing Arctic Ocean: consequences for biological communities, biogeochemical processes and ecosystem functioning'.

An affordable and miniature ice coring drill for rapid acquisition of small iceberg samples.

Icebergs account for approximately half of the freshwater flux from the Greenland Ice Sheet and they can impact marine ecosystems by releasing nutrients and sediments into the ocean as they drift and melt. Parameterizing iceberg fluxes of nutrients and sediments to fjord and ocean waters remains a difficult task due to the complexity of ice-ocean interactions and is complicated by a lack of observations. Acquiring iceberg samples can be difficult and dangerous, as icebergs can break apart and roll without warning. Here we present open source design files for a small, lightweight ice coring drill that can be reproduced using modern computer numerical control (CNC) machining and 3D printing technology. This ice core drill can rapidly acquire small ice samples from icebergs and bergy bits using a standard commercial, off-the-shelf battery-operated hand drill. Design files and a recent field expedition to Northwest Greenland are described. Ice core collection required only 30 s, thereby minimizing risks to scientists.

In situ biodegradation, photooxidation and dissolution of petroleum compounds in Arctic seawater and sea ice.

In pristine sea ice-covered Arctic waters the potential of natural attenuation of oil spills has yet to be uncovered, but increasing shipping and oil exploitation may bring along unprecedented risks of oil spills. We deployed adsorbents coated with thin oil films for up to 2.5 month in ice-covered seawater and sea ice in Godthaab Fjord, SW Greenland, to simulate and investigate in situ biodegradation and photooxidation of dispersed oil. GC-MS-based chemometric methods for oil fingerprinting were used to identify characteristic signatures for dissolution, biodegradation and photooxidation. In sub-zero temperature seawater, fast degradation of n-alkanes was observed with estimated half-life times of ∼7 days. PCR amplicon sequencing and qPCR quantification of bacterial genes showed that a biofilm with a diverse microbial community colonised the oil films, yet a population related to the psychrophilic hydrocarbonoclastic gammaproteobacterium Oleispira antarctica seemed to play a key role in n-alkane degradation. Although Oleispira populations were also present in sea ice, we found that biofilms in sea ice had 25 to 100 times lower bacterial densities than in seawater, which explained the non-detectable n-alkane degradation in sea ice. Fingerprinting revealed that photooxidation, but not biodegradation, transformed polycyclic aromatic compounds through 50 cm-thick sea ice and in the upper water column with removal rates up to ∼1% per day. Overall, our results showed a fast biodegradation of n-alkanes in sea ice-covered seawater, but suggested that oils spills will expose the Arctic ecosystem to bio-recalcitrant PACs over prolonged periods of time.

Adapting open-source drone autopilots for real-time iceberg observations.

Drone autopilots are naturally suited for real-time iceberg tracking as they measure position and orientation (pitch, roll, and heading) and they transmit these data to a ground station. We powered an ArduPilot Mega (APM) 2.6 with a 5V 11 Ah lithium ion battery (a smartphone power bank), placed the APM and battery in a waterproof sportsman's box, and tossed the box and its contents by hand onto an 80 m-long iceberg from an 8 m boat. The data stream could be viewed on a laptop, which greatly enhanced safety while collecting conductivity/temperature/depth (CTD) profiles from the small boat in the iceberg's vicinity. The 10 s position data allowed us to compute the distance of each CTD profile to the iceberg, which is necessary to determine if a given CTD profile was collected within the iceberg's meltwater plume. The APM position data greatly reduced position uncertainty when compared to 5 min position data obtained from a Spot Trace unit. The APM functioned for over 10 h without depleting the battery. We describe the specific hardware used and the software settings necessary to use the APM as a real-time iceberg tracker. Furthermore, the methods described here apply to all Ardupilot-compatible autopilots. Given the low cost ($90) and ease of use, drone autopilots like the APM should be included as another tool for studying iceberg motion and for enhancing safety of marine operations. •Commercial off-the-shelf iceberg trackers are typically configured to record positions over relatively long intervals (months to years) and are not well-suited for short-term (hours to few days), high-frequency monitoring•Drone autopilots are cheap and provide high-frequency (>1 Hz) and real-time information about iceberg drift and orientation•Drone autopilots and ground control software can be easily adapted to studies of iceberg-ocean interactions and operational iceberg management.

Species identification and connectivity of marine amphipods in Canada's three oceans.

Monitoring the distribution of marine biodiversity is a crucial step to better assess the impacts of global changes. Arctic marine fauna is dominated by amphipods in terms of abundance and biomass. These peracarids are an important marine order of crustaceans but the number of species found in the different Canadian oceans is currently unknown. Furthermore, most species are difficult to identify due to poor taxonomic descriptions and morphological convergence. The aim of this study was to assess the species diversity of marine amphipods in the three Canadian oceans using DNA barcoding. To do so, we produced a database of DNA barcodes of amphipods from the three Canadian Oceans publicly available from the BOLD website to which we added 310 new sequences from the Canadian Arctic Archipelago. We first delimited amphipod species based on barcode gap detection techniques and tree based method (bPTP) and then compared the composition of amphipods among the three oceans in order to assess the influence of past transarctic exchanges on Arctic diversity. Our analysis of 2309 sequences which represent more than 250 provisional species revealed a high connectivity between the Atlantic and Arctic Oceans. Our results also suggest that a single threshold to delimitate species is not suitable for amphipods. This study highlights the challenges involved in species delimitation and the need to obtain complete barcoding inventories in marine invertebrates.

Bacterial community succession and degradation patterns of hydrocarbons in seawater at low temperature.

The risk of oil spills in cold marine environments is expected to increase in response to trans-Arctic shipping and as Arctic oil reserves get exploited. Marine hydrocarbon-degrading microbes can reduce the impact of spilled hydrocarbons, but their degradation capabilities at low temperature are yet to be uncovered. We combined DNA amplicon sequencing and chemometrics to investigate the effect of decreasing temperature (0-15 °C) on the succession and function of hydrocarbon-degrading bacteria in seawater. The bacterial community and degradation patterns were investigated at time points when a similar amount of hydrocarbons was mineralised at the different temperatures. This allowed decomposing the effect of temperature into a main component related to the reduced microbial activity at low temperature and a secondary effect. The reduced microbial activity at low temperature delayed the microbial community succession and degradation rates. The secondary effect of temperature was most pronounced at 0 °C, where (1) degradation of the least water-soluble n-alkanes (>C12) was suppressed in contrast to a relative stronger degradation of the most water-soluble n-alkanes (

High geothermal heat flux in close proximity to the Northeast Greenland Ice Stream.

The Greenland ice sheet (GIS) is losing mass at an increasing rate due to surface melt and flow acceleration in outlet glaciers. Currently, there is a large disagreement between observed and simulated ice flow, which may arise from inaccurate parameterization of basal motion, subglacial hydrology or geothermal heat sources. Recently it was suggested that there may be a hidden heat source beneath GIS caused by a higher than expected geothermal heat flux (GHF) from the Earth's interior. Here we present the first direct measurements of GHF from beneath a deep fjord basin in Northeast Greenland. Temperature and salinity time series (2005-2015) in the deep stagnant basin water are used to quantify a GHF of 93 ± 21 mW m-2 which confirm previous indirect estimated values below GIS. A compilation of heat flux recordings from Greenland show the existence of geothermal heat sources beneath GIS and could explain high glacial ice speed areas such as the Northeast Greenland ice stream.